Parallel Sessions

Flow Cytometry Sessions
Electron Microscopy – Biological Science Sessions
Electron Microscopy – Materials Science Sessions
Light Microscopy Sessions



Flow Cytometry Sessions

Amy Baxter

Amy Baxter, CR-CHUM
RNA flow cytometry


Pratip Chattopadhyay

Pratip Chattopadhyay, New York University
Single cell transcriptomics – flow cytometry meets the genomics world

Pratip Chattopadhyay is a Johns Hopkins- and NIH-trained researcher who has been a leader developing and applying polychromatic flow cytometry, immunoassays, and emerging single cell transcriptomic technologies. His accomplishments include reporting the first 18- and 28-color flow cytometry experiments, the first published application of quantum dots and brilliant dyes to flow cytometry, key contributions to data analysis tools for single cell technology, and application of single cell technologies to large biomarker and correlative studies. Pratip recently joined the NYU Cancer Center, where he is responsible for designing and guiding a new cutting-edge human immune monitoring core, and an independent research program. His papers have been cited nearly 4000 times (h-index = 28), and his work appears on the Faculty of 1000, Biolegend, Invitrogen, Fluidigm, and BD Biosciences websites. He has received two NIH Special Service Awards, and was named to the International Society for the Advancement of Cytometry’s “Scholars Program.”


Francois Jean

François Jean, University of British Columbia
Zika and dengue virus and novel antivirals

Dr. François Jean is a tenured associate professor in the field of molecular virology at the University of British Columbia (UBC) Department of Microbiology and Immunology. He is the team leader of a Canada-India Networks of Centres of Excellence cutting-edge research program (NCE IC-IMPACTS) in next generation molecular diagnostic technology for emerging viral diseases such as severe dengue and congenital Zika syndrome.
As the scientific director of the Facility for Infectious Disease and Epidemic Research (FINDER) (2004-2016), Dr. Jean has established at UBC one of the largest university-based Containment Level (CL)-3 facilities in the world [CFI Award ($19.3 million); Dr. Jean co-PI]. He has been leading major international research initiatives funded by CIHR and NCE, including a team grant from the CIHR Pandemic Preparedness Initiative (2008–11) and a newly awarded NCE Canada-India team grant for developing novel diagnostic technology for detecting emerging and re-emerging viruses (http://ic-impacts.com/research/research-projects/dr-francois-jean/).


Joanne Lannigan

Joanne Lannigan, University of Virginia School of Medicine
EV analysis with Imagestream


Alina Lelic

Alina Lelic, McMaster University
McMaster GLP flow facility – Varicella vaccine development


Peter Lopez

Peter Lopez, NYU School of Medicine
Microscopy for flow cytometrists


Desmond Pink

Desmond Pink, University of Alberta
Extracellular Vesicles as cancer biomarkers

Desmond is a Research Associate with Dr. John Lewis (University of Alberta) and scientist in three start-up companies. Educated at both Memorial University of Newfoundland and the University of Alberta, he has spent his career developing and optimizing assays, some of which are commercially available. He is currently focused on the development and validation of "liquid biopsy" to diagnose prostate cancer (and other diseases) using a micro-flow cytometry platform. The team has identified several new markers which are used in their platform to detect and enumerate extracellular vesicles (EVs) which are not only prostate cancer specific, but also indicative of disease status (e.g. normal, benign or aggressive). Working within the Alberta Prostate Cancer Research Initiative (APCaRI), he is currently validating these markers on both retrospective and prospective cohorts.

While his main focus is the development of a liquid biopsy for prostate cancer, Desmond's broad interest lies with assay and platform development for preclinical applications. He has been developing assays to assess EVs for use in tracking the progression of pre-clinical tumor models and strategies for enumerating fluorophore labeled viruses and nanoparticles.


Aja Rieger

Aja Rieger, University of Alberta
Connected component masks to study phagocytosis and the phagocytic synapse

Aja Rieger is the Flow Core Manager for the University of Alberta, Faculty of Medicine and Dentistry. She completed her PhD at the University of Alberta, followed by a post-doctoral fellowship at University of California- Berkeley. Aja Rieger is driven by the fascination of cells and how they work and the fantastic tools at our disposal to study them.


Chris Spring

Chris Spring, St. Michael's Hospital
Technical challenges and solutions in small particle flow cytometry

Chris completed his B.Sc and M.Sc at McMaster University before joining the laboratory of Dr. Heyu Ni at St. Michael's investigating novel mechanisms of thrombus formation. He expanded on this research at the Australian Centre for Blood Diseases at Monash University working with Dr. Shaun Jackson before returning to St. Michael's and joining the RCF. Chris has since developed a special interest in cytometric investigation of extracellular vesicles. Chris has over 10 years of experience in flow cytometry and associated sample techniques, is a certified cytometrist through the International Society for the Advancement of Cytometry (ISAC) and has served with the CCMA since 2012. His work has been published in Nature Cell Biology, Blood, Atherosclerosis Thrombosis and Vascular Biology, the American Journal of Hematology, Nucleic Acids Research, the Journal of Cell Science, Stem Cells Translational Medicine, and Experimental Cell Biology.


Maryam Tabrizian

Maryam Tabrizian, McGill University
Microfluidics/lab-on-a-chip Cell Sorting technology


Vera Tang

Vera Tang, University of Ottawa
Single-particle analysis of virus by flow virometry: Methods for flow cytometer set-up and virus labeling

Vera is the Operations Manager and Research Associate at the University of Ottawa Flow Cytometry & Virometry Core Facility. Her current research interests are in developing methods to characterize and isolate virus populations for analysis using flow cytometers. Viruses of specific interest are HIV and oncolytic viruses such as vaccinia and members of the rhabdovirus family which have relevance in antiviral and cancer therapeutics. In collaboration with researchers at the University of Ottawa and Ottawa Health Research Institute her work on vaccinia virus was recently published in Vaccine.


John Tigges

John Tigges, Beth Israel Deaconess Medical Center
Simplified science with near UV to IR



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Electron Microscopy – Biological Science Sessions

Marcus Brubaker

Marcus Brubaker, York University
Algorithms for reducing the computational burden of cryo-EM

Dr. Marcus Brubaker received his Ph.D. in Computer Science from the University of Toronto. Before joining York University as an Assistant Professor he worked as a postdoctoral researcher at Toyota Technological Institute at Chicago and at University of Toronto, Scarborough. He consults with industry on machine learning and computer vision related projects and has been involved in a number of startups including Cadre Research Labs, itsme3d and Structura Biotechnology. His interests span computer vision, machine learning and statistics and he has worked on a range of problems including video-based human motion estimation, physical models of human motion, Bayesian inference, ballistic forensics, electron cryomicroscopy and autonomous driving.


Marek Malac

Marek Malac, National Institute for Nanotechnology
Phase plate imaging in a transmission electron microscope

Marek Malac is an adjunct professor in Physics at the University of Alberta and a Senior Research Officer with Canada's National Institute for Nanotechnology (NINT), a government-funded research institute run in partnership between Canada's National Research Council (NRC), the University of Alberta and the Government of Alberta—and the hub of nanotechnology R&D in Canada. Marek was responsible for establishing the institute's state-of-the-art electron microscopy lab—currently the largest one in Canada—and helping to develop NINT's new quiet laboratory.

His research focuses on improving our understanding of the physical principles underlying electron microscopy (EM), and developing better instrumentation and methods for data interpretation. In particular, his work focusses on improving phase-retrieval methods in a transmission electron microscope (TEM) and developing experimental methods for quantitative measurement of optical and dispersion relations at nanoscale using electron energy loss spectroscopy (EELS).

Marek also often collaborates with Hitachi High Technologies and JEOL USA, Ltd., on research projects looking at different aspects of EM such as instrument optimization, implementation of new data collection methods and the development of phase-shifting devices.


Joaquin Ortega

Joaquin Ortega, McMaster University
Capturing atomic resolution snapshots of the ribosome assembly process using direct electron detectors

Dr. Joaquin Ortega is currently a Professor in the Department of Biochemistry at McMaster. He received his PhD in Biochemistry in 1999 in Spain. Upon completion of his PhD, Dr. Ortega undertook postdoctoral training at the National Institutes of Health (USA). Currently, his research uses cryo-electron microscopy to understand the structure and molecular mechanisms of protein complexes that perform critical functions in bacterial cells. This is essential information needed to develop new antibiotics and to make existing ones more potent. Dr. Ortega's research team is now moving to the Department of Anatomy and Cell Biology at McGill, the biggest cryo-EM expertise hub in Canada, where he will continue to pursue his research program.


Marek Malac

Martin Parent, Université Laval
The ultrastructural features of serotonin and dopamine axons in parkinsonian monkeys

Dr. Martin Parent is Associate Professor in the Department of Psychiatry and Neuroscience, Faculty of Medicine, Université Laval (Quebec City, Canada). He is also a research group leader at the "Centre de Recherche de l'Intitut Universitaire en Santé Mentale de Québec (CRIUSMQ)". He received his PhD from Université Laval in 2006 and then moved to Montreal to work as a post-doctoral fellow (from 2006 to 2009) with Dr. Laurent Descarries, renowned for his beautiful work on the morphological aspects of neuronal transmission. He then did a short stay at Lund University, in Sweden, to work with Dr. Angela Cenci on animal model of Parkinson's disease and L-Dopa-induced dyskinesia. Since then, he has published many peer-reviewed articles on morphological aspects of serotonin and dopamine transmission in basal ganglia using a wide array of methodological approaches, including transmission electron microscopy.


Martin Pilhofer

Martin Pilhofer, ETH Zurich
Structure, function, and evolution of bacterial contractile injection systems

Martin Pilhofer studied Biology at the University of Bayreuth and at the Technical University Munich. He then joined Karl-Heinz Schleifer's lab for his PhD in Microbiology, investigating the evolution of bacterial cytoskeletal elements and their role in cell division. During his postdoctoral research with Grant Jensen at Caltech/HHMI in Pasadena, Martin discovered bacterial microtubules. Martin then explored the application of electron cryotomography to study bacterial cell-cell interactions, and solved the mechanism of the bacterial Type 6 Secretion System. Since 2014, Martin leads his own lab at ETH Zürich and focuses on the understanding of the structure, function and evolution of bacterial contractile injection systems. Martin was awarded an ERC Starting grant in 2016.


Keana Scott

Keana Scott, National Institute of Standards and Technology
Analyzing nanoparticles using focused ion beam scanning electron microscopy

Keana Scott is a physical scientist at NIST. She has a B.S. in Engineering and Applied Sciences from Caltech and a Ph.D. in Mechanical Engineering from the University of Pittsburgh. After several years working in industry, Keana joined NIST in April 2006 to work on multi-modal bioimaging technique development and the microanalysis of biological materials using electron and ion beams.


Paul Verkade

Paul Verkade, University of Bristol
CLEM, 1 + 1 = 3

Prof. Paul Verkade's research group is based at the University of Bristol where he also heads the EM unit of the Wolfson Bioimaging Facility, a fully integrated light and electron microscopy centre. His research group focuses on the development and application of microscopy techniques mainly for the study of sorting mechanisms in intracellular transport pathways. The main tools are electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which field he has published over 60 papers. Prof. Verkade is currently chair of the Electron Microscopy section of the Royal Microscopy Society. He has also organised and taught on several courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy (CLEM), and immuno EM. His lab is the current home of the EMBO practical course on CLEM (2012, 2014, 2016).



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Electron Microscopy – Materials Science Sessions

Philippe Bocher

Philippe Bocher, ETS Montreal
Measuring Sub-Grain Scale Elastic and Plastic Deformations in Polycrystalline Metallic Alloy by Electron Microscopy Digital Image Correlation

Prof. Bocher (ETS) has developed expertise in the evaluation and simulation of the micromechanical behavior of metallic alloys including deformation, recrystallization, and texture analysis. He also has worked in fracture mechanics and fatigue in the context of industrial alloys. Most of his projects are dedicated to the optimisation of manufacturing processes and part life assessments. In this context, he has developed tools based on digital image correlation to document the local elastic and plasticity behaviour of polycrystals under monotonic deformation or fatigue conditions. These methods have been applied successfully to scanning electron microscopy images providing relevant information on the sub-grain scale behavior of polycrystals under a strain field.


Pierre Hovington

Pierre Hovington, Hydro Quebec
The need and the challenges of material characterization in the development of batteries

  • Undergraduate studies in Physics Engineering, Ecole Polytechnique of Montreal
  • Master degree in the quantification of low energy X-ray at Ecole Polytechnique
  • PhD degree under the co-supervision of Raynald Gauvin of McGill University and Prof D. C.Joy of University of Tennessee and Oak Ridge National laboratory in Monte-Carlo simulation of low Energy Electrons where he co-wrote the famous Casino program
  • Employee from Hydro-Quebec since 1997. He is now the group leader in the characterization department of the Battery project
  • Published over 150 papers in peer reviews journal
  • Co-author of 4 patents on battery materials

Marc Verhaegen

Marc Verhaegen, Photon etc.
Hyperspectral microscope platform for highly multiplex biological imaging

Marc Verhaegen is the Chief Technical Officer of Photon etc, a Montreal based company specialised in optical instruments for academic and industrial research professionals. Marc Verhaegen obtained his Bachelor degree in physics from the Université Catholique de Louvain, his Master's degree in optoelectronics from the Université de Liège, and his Ph.D in physics from the Université de Montréal on the optical properties of silica-based materials. Marc completed his postdoctoral studies in the fibre-optics laboratory at École Polytechnique de Montréal, working specifically on the intensification of second order non-linear effects for fibre-optics and silica glass. Marc Verhaegen is an expert in the manufacture of high-performance devices based on fiber and volume Bragg gratings. He has worked as a researcher and a technical leader in the R&D departments of ITF Optical Technologies and Corvis Canada. He recently joined the administrative board of PRIMA Québec, Pôle de recherche et d'innovation en matériaux avancés du Québec.


Aycan Yurtsever

Aycan Yurtsever, Institut National de la Recherche Scientifique (INRS)
Capturing Materials in Space and Time

Dr. Yurtsever is an Associate Professor at the Énergie Matériaux Télécommunications Center of INRS. He holds a Canada Research Chair in Ultrafast Dynamics of Nanoscale Systems. He received his Ph.D. from Cornell University under the supervision of Prof. David Muller. His post-doctoral appointment was in the group of Prof. Ahmed Zewail (Nobel Laureate in Chemistry) at the California Institute of Technology. There he made significant contributions to the development of advanced imaging methodologies using ultrashort electron and laser pulses. His current research interests include non-equilibrium structural dynamics of 2D materials, ultrafast energy transfer and non-linear optical processes at the nanoscale. He frequently communicates his research results in journals, such as Nano Letters, Physical Review Letters and Science.


Stefan Zaefferer

Stefan Zaefferer, Max-Planck-Institut für Eisenforschung
TBA



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Light Microscopy Sessions

Robert Campbell

Robert Campbell, University of Alberta
Engineering the next generation of genetically encoded reporters of cell signaling activity

Dr. Robert E. Campbell is a Professor in the Department of Chemistry, University of Alberta (2003 - present). He earned his Ph.D. in Chemistry with Martin Tanner at the University of British Columbia in 2000 and undertook postdoctoral research at the University of California San Diego in the lab of the late Roger Y. Tsien, one of the 2008 Nobel Prize winners in Chemistry. His research is focused on the development of optogenetic tools for cell biology applications. His contributions have been recognized with numerous awards including the Teva Canada Limited Biological and Medicinal Chemistry Lectureship Award (2016), the Rutherford Memorial Medal in Chemistry from the Royal Society of Canada (2015), and a Canada Research Chair (2004-2014).


Tohid Didar

Tohid Didar, McMaster University
Microfluidic chips for detection and sorting of primary cells using fluorescence microscopy

Before joining McMaster University in January 2016, Dr. Tohid Didar was a postdoctoral fellow at the Wyss Institute for Biologically Inspired Engineering at Harvard University. There he designed and developed a robust and clinically relevant extracorporeal dialysis-like treatment (DLT) device for sepsis treatment, leveraging medical grade hollow fiber filters. He obtained his PhD degree in 2013 from McGill University. His doctoral research was focused on design and development of miniaturized devices for label-free and high-throughput sorting, detection and in vitro culture of primary and rare cells. His PhD project resulted in 2 patented microfluidic technologies for rare cell detection, separation, patterning and in vitro culture as well as 8 journal publications, 15 conference presentations and several invited talks. During his PhD studies, he was a research fellow at Harvard University, a member of NSERC-Create Integrated Sensor Systems training program and a visiting researcher at the National Research Council of Canada.


Marek Malac

Jesse Greener, Université Laval
A plasmonic/microfluidic hybrid device approach for in situ pH imaging with oral health applications

Jesse Greener has a B.Sc. in chemical physics from the University of Waterloo and a doctorate in physics from the University of Western Ontario. His Ph.D. research focused on the development of time-resolved FTIR to study fundamental interactions between thermally cycled adsorbates on metal surfaces using laser pulsing. In 2011 he launched a successful microfluidic company FlowJEM. Since becoming an assistant professor in the Department of Chemistry at Laval University (2012) he has published nearly 30 papers and book chapter in the area of biomicrofluidics, analytical microfluidics, chemical-imaging and microfabrication.


Sabrina Leslie

Sabrina Leslie, Harvard University
Squeezing new information out of macromolecules using adjustable nanoconfinement

Dr. Leslie received a B.Sc. in Physics and Mathematics from the University of British Columbia in 2002, and received her Ph.D. in Physics from the University of California Berkeley in 2008, where she worked in the Stamper-Kurn lab. She then joined the lab of Adam Cohen at Harvard University where she held the Mary Fieser Postdoctoral Research Fellowship. Her research program creates and applies microscopy instrumentation to enable scientific discoveries in biophysics and materials science research, ranging from fundamental, to applied. To provide microscopic insights into longstanding biological questions, her group invents, engineesr, and machines single-molecule microscopy tools that are newly capable of simultaneously measuring: weak protein-DNA and DNA-DNA interactions; extended search trajectories of individual molecules over several seconds; millisecond-timescale interaction kinetics; and topologically complex or strained DNA substrates.


Nathanaël Prunet

Nathanaël Prunet, Caltech
Patterning the Arabidopsis flower: Superman and the definition of the stamen/carpel boundary

Nat Prunet uses a live confocal microscopy approach to study the development and patterning of the Arabidopsis flower. After a PhD at Ecole Normale Supérieure de Lyon and a postdoc at Dartmouth College, he is now a postdoctoral fellow in the Meyerowitz lab at Caltech. Nat Prunet is fascinated by SciArt and received awards for his microscopy images in multiple competitions including the Nikon Small World, the FASEB BioArt and the Royal Photographic Society's International Images for Science competitions.


Simon Watkins

Simon C. Watkins, University of Pittsburgh Department of Cell Biology
TBA

Dr Watkins is a highly qualified scientist with a extensive pedigree (600 refereed papers, H-Index 123) in the application of cutting edge microscopic imaging to biomedical research. He is a Distinguished Professor and Vice Chair of the department of Cell Biology at the University of Pittsburgh. His current research is focused to developing novel high speed deep tissue imaging approaches for assessing single molecule function in health and disease.

Dr. Watkins is the founder and director of the Center for Biologic Imaging at the University of Pittsburgh, which is in internationally renowned intellectual nexus for the application of all aspects of microscopic imaging specifically for the study of molecular, cellular and tissue biology. He has been successful in nurturing the Center from infancy to a highly productive (35+papers/year), well funded organization employing 25 people including, faculty, postdoctoral fellows and research specialists and students Currently the Center collaborates with over 300 groups within the University and around the country each year.

Dr. Watkins has been successful in attracting funding for his studies using imaging technologies, his current grant income is more than 2 million dollars a year, all of which comes from extramural funding sources Dr. Watkins serves on several NIH study sections each year and chaired 3 such sections in the last year. He directs internationally recognized courses in microscopic imaging at Mount Desert Island Biological laboratories in Maine.

Dr. Watkins is a Fellow of the Royal College of Pathologists, was appointed as the Raine Professor at the University of Western Australia in Perth in 1999 and 2008 and holds an honorary doctorate from the University of Umea in Sweden for his contributions to the field of microscopic imaging.


Moriel Zelikowsky

Moriel Zelikowsky, Caltech
Neuropeptidergic control of an emotion state produced by stress

Dr. Moriel Zelikowsky received her Ph.D. in Psychology (Behavioral Neuroscience) from UCLA in 2011, where she studied the role of the hippocampus in fear learning and memory under the mentorship of Dr. Michael Fanselow. Dr. Zelikowsky continued on to obtain her postdoctoral research training in the lab of Dr. David Anderson in the department of Biology and Biological Engineering at Caltech, where she has been using novel molecular-genetic tools to investigate the neural circuits underlying stress, anxiety, and social behavior.



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